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The three main active ingredients in Neosporin are neomycin sulfate , polymyxin B sulfate , and bacitracin zinc . One of the main components of Neosporin is Neomycin Sulfate which is a type of antibiotic discovered in 1949 by microbiologist Selman Waksman at Rutgers University. [14] Neomycin is a type of aminoglycoside antibiotic that fights against Gram positive and gram negative bacteria . Neomycin is often used in order to prevent risk of bacterial infections. [15] Aminoglycosides such as Neomycin are known for their ability to bind to RNA and to change the proteins being produced by the bacteria with little to no effect on DNA . Neomycin kills bacteria as a result of irregular protein production in the bacterial cell. When the cell can no longer produce the correct proteins, its membrane will be damaged. [16] Like Neomycin, Polymyxin B is an antibiotic. Polymyxin B alters the bacterial cell wall causing the cellular insides to leak out resulting in cell death. This antibiotic also interferes with the production of tetrahydrofolic acid by altering an enzyme. Without the tetrahydrofolic acid, the bacteria can no longer produce proteins necessary for survival. [17] Pramoxine is used to temporarily reduce pain from burns, insect bites, and minor cuts. It works like an anesthetic by decreasing the permeability of neuron membranes. This blocks the ability of pain neurons in the area to send signals which results in numbness. [18]

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